Lesson 6.1: Venepuncture
Venepuncture is one of the most common procedure stations. The technique must be fluid and the communication natural throughout.
Step-by-Step
- Introduce, confirm identity (name, date of birth), explain procedure: 'I need to take a small blood sample from a vein in your arm — it will feel like a sharp scratch.'
- Obtain verbal consent. Ask about needle phobia and previous difficult veins.
- Equipment: vacutainer barrel, needle (21G for most adults), blood bottles in correct order of draw, tourniquet, alcohol wipe, gauze, plaster, sharps bin, gloves.
- Wash hands. Apply gloves.
- Apply tourniquet 5–10 cm above site. Ask patient to clench fist.
- Identify vein — antecubital fossa (median cubital vein) is first choice. Palpate; do not just look.
- Clean skin with alcohol wipe. Allow to dry 30 seconds — do not fan or blow.
- Warn: 'You will feel a sharp scratch now.' Insert needle bevel up at 15–30 degrees. Blood should appear in the hub.
- Attach first vacutainer tube. Allow to fill by vacuum — do not force. Gently invert each tube 5–8 times on removal (do not shake).
- Release tourniquet before withdrawing the needle.
- Withdraw needle. Apply gauze immediately with firm pressure for 2 minutes.
- Activate safety device. Dispose needle directly into sharps bin — never resheath.
- Label bottles at the bedside: patient name, DOB, date, time. Apply plaster.
- Thank the patient. State you would complete the request form and arrange transport to the lab.
Order of Draw — Vacutainer Tubes
| Tube Colour | Contents | Use |
|---|---|---|
| Gold / yellow (SST) | Serum separator | Biochemistry — U&E, LFTs, TFTs, CRP, glucose, HbA1c |
| Green (lithium heparin) | Heparin | Urgent biochemistry |
| Purple / lavender (EDTA) | EDTA | Haematology — FBC, ESR, blood film |
| Blue (citrate) | Citrate | Coagulation — INR, APTT, fibrinogen |
| Grey (fluoride oxalate) | Fluoride + oxalate | Fasting glucose, lactate |
| Pink (EDTA) | EDTA | Group and save / crossmatch — always label in patient's presence |